Enzymatic digestibility and pretreatment degradation products of AFEX-treated hardwoods (Populus nigra).
Identifieur interne : 003410 ( Main/Exploration ); précédent : 003409; suivant : 003411Enzymatic digestibility and pretreatment degradation products of AFEX-treated hardwoods (Populus nigra).
Auteurs : Venkatesh Balan [États-Unis] ; Leonardo Da Costa Sousa ; Shishir P S. Chundawat ; Derek Marshall ; Lekh N. Sharma ; C Kevin Chambliss ; Bruce E. DaleSource :
- Biotechnology progress [ 1520-6033 ]
Descripteurs français
- KwdFr :
- Biotechnologie (méthodes), Cellulase (composition chimique), Cellulose (composition chimique), Endo-1,4-beta xylanases (composition chimique), Glucanes (composition chimique), Hydrolyse (MeSH), Populus (composition chimique), Ressources de production d'énergie (MeSH), Xylanes (composition chimique), Zea mays (composition chimique).
- MESH :
- composition chimique : Cellulase, Cellulose, Endo-1,4-beta xylanases, Glucanes, Populus, Xylanes, Zea mays.
- méthodes : Biotechnologie.
- Hydrolyse, Ressources de production d'énergie.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cellulase, Cellulose, Endo-1,4-beta Xylanases, Glucans, Xylans.
- chemistry : Populus, Zea mays.
- methods : Biotechnology.
- Energy-Generating Resources, Hydrolysis.
Abstract
There is a growing need to find alternatives to crude oil as the primary feed stock for the chemicals and fuel industry and ethanol has been demonstrated to be a viable alternative. Among the various feed stocks for producing ethanol, poplar (Populus nigra x Populus maximowiczii) is considered to have great potential as a biorefinery feedstock in the United States, due to their widespread availability and good productivity in several parts of the country. We have optimized AFEX pretreatment conditions (180 degrees C, 2:1 ammonia to biomass loading, 233% moisture, 30 minutes residence time) and by using various combinations of enzymes (commercical celluloses and xylanases) to achieve high glucan and xylan conversion (93 and 65%, respectively). We have also identified and quantified several important degradation products formed during AFEX using liquid chromatography followed by mass spectrometry (LC-MS/MS). As a part of degradation product analysis, we have also quantified oligosaccharides in the AFEX water wash extracts by acid hydrolysis. It is interesting to note that corn stover (C4 grass) can be pretreated effectively using mild AFEX pretreatment conditions, while on the other hand hardwood poplar requires much harsher AFEX conditions to obtain equivalent sugar yields upon enzymatic hydrolysis. Comparing corn stover and poplar, we conclude that pretreatment severity and enzymatic hydrolysis efficiency are dictated to a large extent by lignin carbohydrate complexes and arabinoxylan cross-linkages for AFEX.
DOI: 10.1002/btpr.160
PubMed: 19326425
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Lansing, MI 48824</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author><author><name sortKey="Sousa, Leonardo Da Costa" sort="Sousa, Leonardo Da Costa" uniqKey="Sousa L" first="Leonardo Da Costa" last="Sousa">Leonardo Da Costa Sousa</name></author><author><name sortKey="Chundawat, Shishir P S" sort="Chundawat, Shishir P S" uniqKey="Chundawat S" first="Shishir P S" last="Chundawat">Shishir P S. Chundawat</name></author><author><name sortKey="Marshall, Derek" sort="Marshall, Derek" uniqKey="Marshall D" first="Derek" last="Marshall">Derek Marshall</name></author><author><name sortKey="Sharma, Lekh N" sort="Sharma, Lekh N" uniqKey="Sharma L" first="Lekh N" last="Sharma">Lekh N. Sharma</name></author><author><name sortKey="Chambliss, C Kevin" sort="Chambliss, C Kevin" uniqKey="Chambliss C" first="C Kevin" last="Chambliss">C Kevin Chambliss</name></author><author><name sortKey="Dale, Bruce E" sort="Dale, Bruce E" uniqKey="Dale B" first="Bruce E" last="Dale">Bruce E. Dale</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009 Mar-Apr</date><idno type="RBID">pubmed:19326425</idno><idno type="pmid">19326425</idno><idno type="doi">10.1002/btpr.160</idno><idno type="wicri:Area/Main/Corpus">003604</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003604</idno><idno type="wicri:Area/Main/Curation">003604</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003604</idno><idno type="wicri:Area/Main/Exploration">003604</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enzymatic digestibility and pretreatment degradation products of AFEX-treated hardwoods (Populus nigra).</title><author><name sortKey="Balan, Venkatesh" sort="Balan, Venkatesh" uniqKey="Balan V" first="Venkatesh" last="Balan">Venkatesh Balan</name><affiliation wicri:level="4"><nlm:affiliation>Biomass Conversion Research Laboratory, Dept. of Chemical Engineering and Materials Science, Michigan State University, E. Lansing, MI 48824, USA. balan@msu.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biomass Conversion Research Laboratory, Dept. of Chemical Engineering and Materials Science, Michigan State University, E. Lansing, MI 48824</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author><author><name sortKey="Sousa, Leonardo Da Costa" sort="Sousa, Leonardo Da Costa" uniqKey="Sousa L" first="Leonardo Da Costa" last="Sousa">Leonardo Da Costa Sousa</name></author><author><name sortKey="Chundawat, Shishir P S" sort="Chundawat, Shishir P S" uniqKey="Chundawat S" first="Shishir P S" last="Chundawat">Shishir P S. Chundawat</name></author><author><name sortKey="Marshall, Derek" sort="Marshall, Derek" uniqKey="Marshall D" first="Derek" last="Marshall">Derek Marshall</name></author><author><name sortKey="Sharma, Lekh N" sort="Sharma, Lekh N" uniqKey="Sharma L" first="Lekh N" last="Sharma">Lekh N. Sharma</name></author><author><name sortKey="Chambliss, C Kevin" sort="Chambliss, C Kevin" uniqKey="Chambliss C" first="C Kevin" last="Chambliss">C Kevin Chambliss</name></author><author><name sortKey="Dale, Bruce E" sort="Dale, Bruce E" uniqKey="Dale B" first="Bruce E" last="Dale">Bruce E. Dale</name></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biotechnology (methods)</term><term>Cellulase (chemistry)</term><term>Cellulose (chemistry)</term><term>Endo-1,4-beta Xylanases (chemistry)</term><term>Energy-Generating Resources (MeSH)</term><term>Glucans (chemistry)</term><term>Hydrolysis (MeSH)</term><term>Populus (chemistry)</term><term>Xylans (chemistry)</term><term>Zea mays (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biotechnologie (méthodes)</term><term>Cellulase (composition chimique)</term><term>Cellulose (composition chimique)</term><term>Endo-1,4-beta xylanases (composition chimique)</term><term>Glucanes (composition chimique)</term><term>Hydrolyse (MeSH)</term><term>Populus (composition chimique)</term><term>Ressources de production d'énergie (MeSH)</term><term>Xylanes (composition chimique)</term><term>Zea mays (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulase</term><term>Cellulose</term><term>Endo-1,4-beta Xylanases</term><term>Glucans</term><term>Xylans</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulase</term><term>Cellulose</term><term>Endo-1,4-beta xylanases</term><term>Glucanes</term><term>Populus</term><term>Xylanes</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Energy-Generating Resources</term><term>Hydrolysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Hydrolyse</term><term>Ressources de production d'énergie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is a growing need to find alternatives to crude oil as the primary feed stock for the chemicals and fuel industry and ethanol has been demonstrated to be a viable alternative. Among the various feed stocks for producing ethanol, poplar (Populus nigra x Populus maximowiczii) is considered to have great potential as a biorefinery feedstock in the United States, due to their widespread availability and good productivity in several parts of the country. We have optimized AFEX pretreatment conditions (180 degrees C, 2:1 ammonia to biomass loading, 233% moisture, 30 minutes residence time) and by using various combinations of enzymes (commercical celluloses and xylanases) to achieve high glucan and xylan conversion (93 and 65%, respectively). We have also identified and quantified several important degradation products formed during AFEX using liquid chromatography followed by mass spectrometry (LC-MS/MS). As a part of degradation product analysis, we have also quantified oligosaccharides in the AFEX water wash extracts by acid hydrolysis. It is interesting to note that corn stover (C4 grass) can be pretreated effectively using mild AFEX pretreatment conditions, while on the other hand hardwood poplar requires much harsher AFEX conditions to obtain equivalent sugar yields upon enzymatic hydrolysis. Comparing corn stover and poplar, we conclude that pretreatment severity and enzymatic hydrolysis efficiency are dictated to a large extent by lignin carbohydrate complexes and arabinoxylan cross-linkages for AFEX.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19326425</PMID><DateCompleted><Year>2009</Year><Month>06</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>2</Issue><PubDate><MedlineDate>2009 Mar-Apr</MedlineDate></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Enzymatic digestibility and pretreatment degradation products of AFEX-treated hardwoods (Populus nigra).</ArticleTitle><Pagination><MedlinePgn>365-75</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.160</ELocationID><Abstract><AbstractText>There is a growing need to find alternatives to crude oil as the primary feed stock for the chemicals and fuel industry and ethanol has been demonstrated to be a viable alternative. Among the various feed stocks for producing ethanol, poplar (Populus nigra x Populus maximowiczii) is considered to have great potential as a biorefinery feedstock in the United States, due to their widespread availability and good productivity in several parts of the country. We have optimized AFEX pretreatment conditions (180 degrees C, 2:1 ammonia to biomass loading, 233% moisture, 30 minutes residence time) and by using various combinations of enzymes (commercical celluloses and xylanases) to achieve high glucan and xylan conversion (93 and 65%, respectively). We have also identified and quantified several important degradation products formed during AFEX using liquid chromatography followed by mass spectrometry (LC-MS/MS). As a part of degradation product analysis, we have also quantified oligosaccharides in the AFEX water wash extracts by acid hydrolysis. It is interesting to note that corn stover (C4 grass) can be pretreated effectively using mild AFEX pretreatment conditions, while on the other hand hardwood poplar requires much harsher AFEX conditions to obtain equivalent sugar yields upon enzymatic hydrolysis. Comparing corn stover and poplar, we conclude that pretreatment severity and enzymatic hydrolysis efficiency are dictated to a large extent by lignin carbohydrate complexes and arabinoxylan cross-linkages for AFEX.</AbstractText><CopyrightInformation>(c) 2009 American Institute of Chemical Engineers Biotechnol.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Balan</LastName><ForeName>Venkatesh</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Biomass Conversion Research Laboratory, Dept. of Chemical Engineering and Materials Science, Michigan State University, E. Lansing, MI 48824, USA. balan@msu.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sousa</LastName><ForeName>Leonardo da Costa</ForeName><Initials>Lda C</Initials></Author><Author ValidYN="Y"><LastName>Chundawat</LastName><ForeName>Shishir P S</ForeName><Initials>SP</Initials></Author><Author ValidYN="Y"><LastName>Marshall</LastName><ForeName>Derek</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Sharma</LastName><ForeName>Lekh N</ForeName><Initials>LN</Initials></Author><Author ValidYN="Y"><LastName>Chambliss</LastName><ForeName>C Kevin</ForeName><Initials>CK</Initials></Author><Author ValidYN="Y"><LastName>Dale</LastName><ForeName>Bruce E</ForeName><Initials>BE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005936">Glucans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.8</RegistryNumber><NameOfSubstance UI="D043364">Endo-1,4-beta Xylanases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043364" MajorTopicYN="N">Endo-1,4-beta Xylanases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004736" MajorTopicYN="N">Energy-Generating Resources</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="N">Glucans</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014990" MajorTopicYN="N">Xylans</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>3</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>3</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>6</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19326425</ArticleId><ArticleId IdType="doi">10.1002/btpr.160</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><noCountry><name sortKey="Chambliss, C Kevin" sort="Chambliss, C Kevin" uniqKey="Chambliss C" first="C Kevin" last="Chambliss">C Kevin Chambliss</name><name sortKey="Chundawat, Shishir P S" sort="Chundawat, Shishir P S" uniqKey="Chundawat S" first="Shishir P S" last="Chundawat">Shishir P S. Chundawat</name><name sortKey="Dale, Bruce E" sort="Dale, Bruce E" uniqKey="Dale B" first="Bruce E" last="Dale">Bruce E. Dale</name><name sortKey="Marshall, Derek" sort="Marshall, Derek" uniqKey="Marshall D" first="Derek" last="Marshall">Derek Marshall</name><name sortKey="Sharma, Lekh N" sort="Sharma, Lekh N" uniqKey="Sharma L" first="Lekh N" last="Sharma">Lekh N. Sharma</name><name sortKey="Sousa, Leonardo Da Costa" sort="Sousa, Leonardo Da Costa" uniqKey="Sousa L" first="Leonardo Da Costa" last="Sousa">Leonardo Da Costa Sousa</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Balan, Venkatesh" sort="Balan, Venkatesh" uniqKey="Balan V" first="Venkatesh" last="Balan">Venkatesh Balan</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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